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Starscream is the secondary antagonist of the Transformers "Aligned" universe. He is the treacherous (former) second-in-command of the Decepticons who initially seeks to usurp control of the Decepticons from Megatron,

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           Transformers: Prime

Autobots
Team Prime
Optimus Prime | Bumblebee | Arcee | Bulkhead | Ratchet | Wheeljack | Smokescreen | Ultra MagnusKnock Out
Cybertron-based
Sentinel Zeta PrimeIronhideJetfireSideswipe • Air Raid • WarpathSilverbolt • Perceptor • GrimlockSlugSwoopSnarlSludgeMetroplex
Bee Team
Strongarm • Sideswipe • Fixit • GrimlockDrift • Slipstream • Jetstorm
Allies
WindbladeJazz • Undertone • Blastwave • Blurr

Others
Humans
Jack DarbyMiko NakadaiRafael EsquivelWilliam Fowler • Denny Clay • Russell Clay
Cybertronian
Hi-Test • Cyberwarp
Weaponizer Mini-Cons
Aerobolt • Buzzstrike • Sawtooth • Tricerashot • Lancelon • Windstrike • Bashbreaker

Decepticons
Leadership
MegatronStarscreamSoundwave
Unicron
Unicron • Terrorcons
Other
PredakingPredacons
Mercenaries: Shadelock

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